Applications of Stem cells and Regenerative Medicine

2^nd World Congress on Stem Cells and Regenerative Medicine

Date: March 14-15, 2019

Place: London, UK

Link: http://stemcells.alliedacademies.com/europe

Applications of Stem cells and Regenerative Medicine

Hematopoietic stem cell transplantation (HSCT) is a specialized intervention performed at selected centres across the world. The extent to which specific aspects of care in allogeneic HSCT have been studied and the types of studies performed for different aspects of care remains incompletely different. Studies in allogeneic HSCT were systematically identified from selected high-profile transplant journals between the period July 2010 and June 2011 and previously reported in a study addressing the definition of clinical outcomes in HSCT. All articles were retrieved and assessed for study characteristics and categorized by specific aspects of care related to allogeneic HSCT.

The most studied aspect of care was conditioning regimens. Transfusion practices were the most understudied aspect of care. Interestingly, most studies included both adult and pediatric patients. Studies involving all hematological malignancies were encountered more often than disease-specific studies. Geographically, most patients described in the published reports were treated only in North America or only in Europe.

 

Most studies were retrospective (78), and 25 reported on multicenter registry data. Of the 38 prospective studies, 8 were randomized controlled trials (RCTs) and predominantly focused on prevention and treatment of graft-versus-host disease (GVHD) and infections. Median follow-up was longer in retrospective registry studies (54 months) and shortest in RCTs (32 months). The proportion of positive outcomes in retrospective and prospective studies was remarkably high (>80% for all categories) and not significantly different across all aspects of care (P > .05). When comparing RCTs and registry data studies, this proportion was similar and high (95% and 100%, respectively, P > .05). Our study highlights the established and important role of retrospective registry studies for many aspects of care and suggests RCTs may be most relevant for studies on infectious complications and GVHD.

Join the talk here: http://stemcells.alliedacademies.com/europe/registration

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Contact: Jessie Reyes
T: 44 203 769 1755[Kindly mention Jessie Reyes/ Helen]
W: (44) 140 358 2077
E: stemcellcongress[at]geneconferences[dot]com

Applications of Stem cells and Regenerative Medicine

2^ns World Congress on Stem Cells and Regenerative Medicine

Date: March 14-15, 2019

Place: London, UK

Link: http://stemcells.alliedacademies.com/europe

Applications of Stem cells and Regenerative Medicine

Stem cells and Regenerative medicine is the most recent and emerging branch of medical science which deals with functional restoration of tissues or organs for the patient suffering from severe injuries or chronic disease or fracture. The spectacular progress in the field of stem cell research has laid the foundation for cell-based therapies in which the diseases cannot be cured by conventional medicines. The indefinite self-renewal and potential to differentiate into other types of cells Laid the way for stem cells as frontiers of regenerative medicine. The transdifferentiating potential of stem cells varies with sources and according to that regenerative applications also change. Advancements in gene editing and tissue engineering technology have endorsed the ex vivo remodelling of stem cells grown into 3D organoids and tissue structures for personalized applications.

 

This review outlines most of the recent advancement in transplantation and therapies in tissue engineering technologies of ESCs, TSPSCs, MSCs, UCSCs, BMSCs, and iPSCs. Additionally, stem cells regenerative application is also discussed in wildlife conservation.

Join the talk here: http://stemcells.alliedacademies.com/europe/registration

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Contact: Jessie Reyes
T: 44 203 769 1755[Kindly mention Jessie Reyes/ Helen]
W: (44) 140 358 2077
E: stemcellcongress[at]geneconferences[dot]com

Heidi Abrahamse presenting 'Potential Use of Photobiomodulation In Stem Cell Therapy' at London

2^ns World Congress on Stem Cells and Regenerative Medicine

Date: March 14-15, 2019

Place: London, UK

Link: http://stemcells.alliedacademies.com/europe

Potential Use of Photobiomodulation In Stem Cell Therapy

Regenerative medicine and tissue engineering (TE), combines key elements such as biomaterials, stem cells and bioactive agents (e.g. growth factors), in parallel with recent biotechnological advances. A constant and reliable source of autologous stem cells with pluripotent potential and readily available will be required for these future cell-based. Bone marrow stem cells (BMSCs) have been extensively studied but clinical application of these cells has presented problems including low cell number upon harvest, pain and morbidity to the donor. Adipose tissue is derived from the mesenchyme and contains an easily isolated supportive stroma containing stem cells, microvascular endothelial cells and smooth muscle cells. Adipose derived stem cells (ADSCs) isolated from adipose tissue are isolated with ease and in large amounts. Stem cells have two major characteristics of self-renewal and differentiation into one or more types of specialised cells.

  

These cells are now being used to treat several degenerative diseases due to their ability to differentiate into different cell types. Photobiomodulation (PBM) or low intensity laser irradiation (LILI) can positively affect human ADSCs by increasing cellular proliferation, viability and protein expression. These characteristics improves their potential in TE applications as the initial cell number could be increased before commencing differentiation leading to a higher yield of differentiated cells. Photobiomodulation at different intensities has been shown to inhibit as well as stimulate cellular processes. Studies on photobiomodulation and stem cells have shown that low-level lasers increase adenosine triphosphate (ATP) production, and migration. Photobiomodulation also promote the proliferation of rat mesenchymal bone marrow and cardiac stem cells in vitro and can thus be used to stimulate the in vitro production of higher stem cell numbers. The addition of specific growth factors could enhance the differentiation of the stem cells into different cell types that could, in turn, be used in TE applications and reconstructive surgery.

However, to be effective for use in TE, certain criteria need to be met including that the cells of interest must be at high concentrations, harvested easily and be multipotent while being able to differentiate into the required tissue and then transplanted safely and effectively back into a host. The potential augmentation of low intensity laser irradiation on ADSCs to differentiate into smooth muscle cells (SMCs) with the view of using as therapeutic modality in regenerative medicine has been the key focus of our research. The effect of different wavelengths of irradiation, as well as different fluences were used to identify laser parameters affecting viability and proliferation of ADSCs. Throughout the project, suitably recognized stem cell markers were used to characterize and confirm stem cells as well as potential differentiation. In addition, dose responses were performed to determine suitable growth factors and concentrations that would induce differentiation. Our results confirm that laser irradiation induce increased viability and proliferation as well as improve the differentiation potential of ADSCs. Methodology used include, ATP content and optical density, flow cytometry, fluorescence microscopy and real-time quantitative polymerase chain reaction RT- qPCR profiles. This further highlights the significant role that LILI has to offer in the use of ADSC therapy in regenerative medicine.

Join the talk here: http://stemcells.alliedacademies.com/europe/registration

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Contact: Jessie Reyes
T: 44 203 769 1755[Kindly mention Jessie Reyes/ Helen]
W: (44) 140 358 2077
E: stemcellcongress[at]geneconferences[dot]com

Alok Kumar presenting "Regeneration of liver by Platycladus orientalis extract" at London, UK

2^ns World Congress on Stem Cells and Regenerative Medicine

Date: March 14-15, 2019

Place: London, UK

Link: http://stemcells.alliedacademies.com/europe

Regeneration of liver by Platycladus orientalis extract

Liver is a vital organ of our body performing wide range of functions. Regeneration of liver by through plant extract is safe and having better patient compliance. Present study was aimed at evaluating protective ability of Platycladus orientalis Linn against paracetamol induced hepatotoxicity in rats. When these extracts screened for hepatoprotective activity, the results obtained from paracetamol induced hepatotoxic model in the healthy male Wister rats indicates that, after the treatment with paracetamol there was significant rise in SGOT, SGPT, ALP and total bilirubin levels which were lowered by petroleum ether extract of Platycladus orientalis leaf, sylimarin 25 mg/kg was taken as the standard drug. In the whole process of hepatotoxicity thioacetamide was used for toxified the liver and it showed same result as like paracetamol in toxification.

 

In order to detect the oxidative stress in lipid peroxidation in liver checked it’s in-vivo antioxidant parameter like reduced glutathione (GSH), thiobarbituric acid reactive substances (TBARS), catalase (CAT) and superoxide dismutase (SOD) which justify the antioxidant property of both the plant. Which also justify the hepatoprotective and antioxidant effect of the plant. The biochemical observations were supplemented by histopathological examination of pancreas and liver section. In histopathological observation, it was showed that the petroleum ether extract of Platycladus orientalis possess effectively hepatoprotective property

Join the talk here: http://stemcells.alliedacademies.com/europe/registration

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Contact: Jessie Reyes
T: 44 203 769 1755[Kindly mention Jessie Reyes/ Helen]
W: (44) 140 358 2077
E: stemcellcongress[at]geneconferences[dot]com

2nd World Congress on Stem Cells and Regenerative Medicine

2^nd World Congress on Stem Cells and Regenerative Medicine

Date: March 14-15, 2019

Place: London, UK

Link: http://stemcells.alliedacademies.com/europe

Laser-induced differentiation of immortalized adipose stem cells to neuronal cells

Adult stem cells of mesodermal origin differentiate into other tissues such as cartilage and bone when treated with specialised induction media in vitro. However, transdifferentiating Adipose Stem Cells (ASCs) to other dermal layers is a challenge to researchers and therapists in regenerative medicine.

Current activities in phototherapy are focused in optimizing the biological activities of lasers or light on stem cells including human immortalized ASCs (iASCs). A growing body of literature suggests that Low Intensity Laser Irradiation (LILI) increase stem cell migration, stimulates proliferation and possibly differentiates them to other cell types. This study used a combination of biological and physical inducers for increasing the differentiation of neurons in culture models. It has used a combination of growth inducers to differentiate iASCs to free-floating neural stem cells called neurospheres.

Further, it has applied Near Infrared (NIR) lasers of wavelength 825 nm with fluences ranging from 5 to 15 J/cm2 on these neurospheres. Changes in the metabolic and redox status of these newly differentiated neurons were gauged from transcriptome. Moreover, neuronal differentiation was determined by immunostaining using early and late markers. This study was able to generate neurospheres from iASCs and differentiate them to neuronal cells in vitro.

There was a sharp distinction between the metabolic processes of these iASCs with the primary ASCs. Strikingly, there was an increase in an early neuronal marker at 5 J/cm2 and 15 J/cm2 signifying the biphasic dose response of NIR laser on living systems. Thus, LILI increased the yield of neurons and effected stem cell differentiation through modulation of cellular redox status.

However, these differentiated cells failed to express late neuronal markers. This study found that iASCs, which has the capacity to proliferate indefinitely in culture medium is an excellent model for differentiation. It gives an insight into the cellular and molecular events during neuronal differentiation of iASCs by growth factors and LILI. Further, it has identified the mode of action of NIR laser in differentiating iASCs to other cell types. The outcome of this study has to be taken forward for validation by functionality testing and analysis.

Join the talk here: http://stemcells.alliedacademies.com/europe/registration

Avail the 50% group benefits

Contact: 

Jessie Reyes
T: 44 203 769 1755[Kindly mention Jessie Reyes/ Helen]
W: (44) 140 358 2077
E: stemcellcongress[at]geneconferences[dot]com